Gender is a remarkable, socially basic concept got from appearances and voices, yet the cerebrum forms hidden gender segregation have not been greatly investigated. The current study investigated male and female voices and the difference between their pitch. To fulfill, 26 male (n=23) and female (n=23) advance EFL learners were selected from a private English language institute. Firstly, the measured brain response differences were observed between female and male voices beginning at 93 ms. Then, using normal, high- and low-pitch voices, pitch differences was checked between both genders. The findings revealed early effects (31–74 ms) was made by differences in pitch. Gender impacts were viewed with implicit pitch processing, but were not viewed with utilization of pitch. Moreover, stage between 191 and 276 ms differentiated male from female voices, independent of pitch. Thus, these data indicated that voice gender processing included two phases; a very early pitch or frequency distinction and a later more precise distinction of gender.